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KMID : 1190620110070010018
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Journal of the Korean Wound Care Society
2011 Volume.7 No. 1 p.18 ~ p.24
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Effect of Human Hypertrophic Scar Fibroblasts Allograft on Proliferation and Collagen Synthesis of Diabetic Fibroblasts In Vitro: A Pilot Study
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Lee Hyup-Woo
Han Seung-Kyu
Wang Chang-Yin
Ko Yong
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Abstract
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The purpose of this pilot study was designed to compare the effect of allograft of human hypertrophic scar fibroblasts with that of normal fibroblasts on the activity of diabetic fibroblasts in vitro. Human hypertrophic scar fibroblasts and normal fibroblasts from the same patients were seeded in 24-well culture inserts. Diabetic fibroblasts were cultured on the bottom 24-well culture plates. Three groups were evaluated, group I: diabetic fibroblasts only, group II: diabetic fibroblasts co-cultured with normal fibroblasts, group III: diabetic fibroblasts co-cultured with human hypertrophic scar fibroblasts. After 3-day incubation, cell proliferation and collagen synthesis, which are the major contributing factors in wound healing, were measured. Group III was the highest level in cell proliferation (P＜0.05), and collagen synthesis demonstrate also highest but the P-value was more than 0.05. The FGFb and VEGF assay did not show consistent results.
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KEYWORD
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Diabetic fibroblast, Hypertrophic scar fibroblast, Co-culture, Collagen type I
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